<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03204120</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-07T04:18:05+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Virulent Shigella flexneri Affects Secretion, Expression, and Glycosylation of Gel-Forming Mucins in Mucus-Producing Cells</title>
            <author role="aut">
              <persName>
                <forename type="first">Brice</forename>
                <surname>Sperandio</surname>
              </persName>
              <idno type="halauthorid">948075-0</idno>
              <affiliation ref="#struct-30757"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Natalie</forename>
                <surname>Fischer</surname>
              </persName>
              <idno type="halauthorid">1061804-0</idno>
              <affiliation ref="#struct-30757"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Marie Joncquel</forename>
                <surname>Chevalier-Curt</surname>
              </persName>
              <idno type="halauthorid">2200606-0</idno>
              <affiliation ref="#struct-1330"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Yannick</forename>
                <surname>Rossez</surname>
              </persName>
              <email type="md5">71ed65219398929babfc176150dd3a73</email>
              <email type="domain">univ-lille.fr</email>
              <idno type="idhal" notation="string">yannick-rossez</idno>
              <idno type="idhal" notation="numeric">739000</idno>
              <idno type="halauthorid" notation="string">13579-739000</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5435-1373</idno>
              <idno type="IDREF">https://www.idref.fr/164273875</idno>
              <affiliation ref="#struct-1330"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Pascal</forename>
                <surname>Roux</surname>
              </persName>
              <idno type="halauthorid">121394-0</idno>
              <affiliation ref="#struct-18375"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Catherine</forename>
                <surname>Robbe</surname>
              </persName>
              <email type="md5">ec9f5c75f741fdf7d83b86c6f3ad10a4</email>
              <email type="domain">univ-lille.fr</email>
              <idno type="idhal" notation="string">catherine-robbe-masselot</idno>
              <idno type="idhal" notation="numeric">183875</idno>
              <idno type="halauthorid" notation="string">2646-183875</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6266-6033</idno>
              <idno type="IDREF">https://www.idref.fr/07670663X</idno>
              <affiliation ref="#struct-1330"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Philippe</forename>
                <surname>Sansonetti</surname>
              </persName>
              <email type="md5">641bc95a151ff144f51b06d3146b849b</email>
              <email type="domain">pasteur.fr</email>
              <idno type="idhal" notation="numeric">957753</idno>
              <idno type="halauthorid" notation="string">841048-957753</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-7542-4527</idno>
              <idno type="IDREF">https://www.idref.fr/060758082</idno>
              <affiliation ref="#struct-30757"/>
              <affiliation ref="#struct-229965"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>LillOA</forename>
                <surname>Université de Lille</surname>
              </persName>
              <email type="md5">07a16304e296c6e387b401d984336838</email>
              <email type="domain">univ-lille.fr</email>
            </editor>
            <funder ref="#projeurop-91946"/>
            <funder ref="#projeurop-13551"/>
            <funder>The research leading to these results has received funding from the European Union Seventh Framework Programme under grant agreement EIMID ITN no. 264388. P. J. Sansonetti is supported by the European Research Council (HOMEOEPITH project) and by the Howard Hughes Medical Institute.</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2021-04-21 12:12:43</date>
              <date type="whenModified">2026-05-07 03:25:25</date>
              <date type="whenReleased">2021-04-21 12:12:43</date>
              <date type="whenProduced">2013-10-01</date>
              <ref type="externalLink" target="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3811771"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="653105">
                <persName>
                  <forename>LillOA</forename>
                  <surname>Université de Lille</surname>
                </persName>
                <email type="md5">07a16304e296c6e387b401d984336838</email>
                <email type="domain">univ-lille.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03204120</idno>
            <idno type="halUri">https://lilloa.hal.science/hal-03204120</idno>
            <idno type="halBibtex">sperandio:hal-03204120</idno>
            <idno type="halRefHtml">&lt;i&gt;Infection and Immunity&lt;/i&gt;, 2013, Infection and immunity, 81 (10), pp.3632-3643. &lt;a target="_blank" href="https://dx.doi.org/10.1128/IAI.00551-13"&gt;&amp;#x27E8;10.1128/IAI.00551-13&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Infection and Immunity, 2013, Infection and immunity, 81 (10), pp.3632-3643. &amp;#x27E8;10.1128/IAI.00551-13&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
            <idno type="stamp" n="PASTEUR">Institut Pasteur</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="CDF">Collège de France</idno>
            <idno type="stamp" n="OPENAIRE">OpenAIRE</idno>
            <idno type="stamp" n="PSL">Université Paris sciences et lettres</idno>
            <idno type="stamp" n="UNIV-LILLE">Université de Lille</idno>
            <idno type="stamp" n="CDF-PSL" corresp="PSL">Collège de France - PSL </idno>
            <idno type="stamp" n="ACADEMIE-SCIENCES-PUBLICATIONS-MEMBRES" corresp="ACADEMIE-SCIENCES">Publications des membres, correspondants et associés étrangers de l'Académie des sciences</idno>
            <idno type="stamp" n="ACADEMIE-SCIENCES-PUBLICATIONS-MEMBRES-2" corresp="ACADEMIE-SCIENCES-PUBLICATIONS-MEMBRES">Publications des membres de l'Académie des sciences (lot 2)</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="1">Not set</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Virulent Shigella flexneri Affects Secretion, Expression, and Glycosylation of Gel-Forming Mucins in Mucus-Producing Cells</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Brice</forename>
                    <surname>Sperandio</surname>
                  </persName>
                  <idno type="halauthorid">948075-0</idno>
                  <affiliation ref="#struct-30757"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Natalie</forename>
                    <surname>Fischer</surname>
                  </persName>
                  <idno type="halauthorid">1061804-0</idno>
                  <affiliation ref="#struct-30757"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Marie Joncquel</forename>
                    <surname>Chevalier-Curt</surname>
                  </persName>
                  <idno type="halauthorid">2200606-0</idno>
                  <affiliation ref="#struct-1330"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Yannick</forename>
                    <surname>Rossez</surname>
                  </persName>
                  <email type="md5">71ed65219398929babfc176150dd3a73</email>
                  <email type="domain">univ-lille.fr</email>
                  <idno type="idhal" notation="string">yannick-rossez</idno>
                  <idno type="idhal" notation="numeric">739000</idno>
                  <idno type="halauthorid" notation="string">13579-739000</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5435-1373</idno>
                  <idno type="IDREF">https://www.idref.fr/164273875</idno>
                  <affiliation ref="#struct-1330"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Pascal</forename>
                    <surname>Roux</surname>
                  </persName>
                  <idno type="halauthorid">121394-0</idno>
                  <affiliation ref="#struct-18375"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Catherine</forename>
                    <surname>Robbe</surname>
                  </persName>
                  <email type="md5">ec9f5c75f741fdf7d83b86c6f3ad10a4</email>
                  <email type="domain">univ-lille.fr</email>
                  <idno type="idhal" notation="string">catherine-robbe-masselot</idno>
                  <idno type="idhal" notation="numeric">183875</idno>
                  <idno type="halauthorid" notation="string">2646-183875</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6266-6033</idno>
                  <idno type="IDREF">https://www.idref.fr/07670663X</idno>
                  <affiliation ref="#struct-1330"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Philippe</forename>
                    <surname>Sansonetti</surname>
                  </persName>
                  <email type="md5">641bc95a151ff144f51b06d3146b849b</email>
                  <email type="domain">pasteur.fr</email>
                  <idno type="idhal" notation="numeric">957753</idno>
                  <idno type="halauthorid" notation="string">841048-957753</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-7542-4527</idno>
                  <idno type="IDREF">https://www.idref.fr/060758082</idno>
                  <affiliation ref="#struct-30757"/>
                  <affiliation ref="#struct-229965"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">14165</idno>
                <idno type="issn">0019-9567</idno>
                <idno type="eissn">1098-5522</idno>
                <title level="j">Infection and Immunity</title>
                <imprint>
                  <publisher>American Society for Microbiology</publisher>
                  <biblScope unit="serie">Infection and immunity</biblScope>
                  <biblScope unit="volume">81</biblScope>
                  <biblScope unit="issue">10</biblScope>
                  <biblScope unit="pp">3632-3643</biblScope>
                  <date type="datePub">2013-10-01</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1128/IAI.00551-13</idno>
              <idno type="pubmed">23876800</idno>
              <idno type="pubmedcentral">PMC3811771</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="mesh">Electrolytes</classCode>
              <classCode scheme="mesh">Endoplasmic Reticulum Stress</classCode>
              <classCode scheme="mesh">Virulence</classCode>
              <classCode scheme="mesh">Gels</classCode>
              <classCode scheme="mesh">Gene Expression Regulation</classCode>
              <classCode scheme="mesh">HT29 Cells</classCode>
              <classCode scheme="mesh">Humans</classCode>
              <classCode scheme="mesh">Mucins</classCode>
              <classCode scheme="mesh">Peptides</classCode>
              <classCode scheme="mesh">Shigella flexneri</classCode>
              <classCode scheme="mesh">Trefoil Factor-2</classCode>
              <classCode scheme="halDomain" n="chim.theo">Chemical Sciences/Theoretical and/or physical chemistry</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Mucin glycoproteins are secreted in large amounts by the intestinal epithelium and constitute an efficient component of innate immune defenses to promote homeostasis and protect against enteric pathogens. In this study, our objective was to investigate how the bacterial enteropathogen Shigella flexneri, which causes bacillary dysentery, copes with the mucin defense barrier. We report that upon in vitro infection of mucin-producing polarized human intestinal epithelial cells, virulent S. flexneri manipulates the secretion of gel-forming mucins. This phenomenon, which is triggered only by virulent strains, results in accumulation of mucins at the cell apical surface, leading to the appearance of a gel-like structure that favors access of bacteria to the cell surface and the subsequent invasion process. We identify MUC5AC, a gel-forming mucin, as a component of this structure. Formation of this gel does not depend on modifications of electrolyte concentrations, induction of trefoil factor expression, endoplasmic reticulum stress, or response to unfolded proteins. In addition, transcriptional and biochemical analyses of infected cells reveal modulations of mucin gene expression and modifications of mucin glycosylation patterns, both of which are induced by virulent bacteria in a type III secretion system-dependent manner. Thus, S. flexneri has developed a dedicated strategy to alter the mucus barrier by targeting key elements of the gel-forming capacity of mucins: gene transcription, protein glycosylation, and secretion.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-30757" status="OLD">
          <orgName>Pathogénie Microbienne Moléculaire</orgName>
          <date type="start">2006-01-01</date>
          <date type="end">2014-12-31</date>
          <desc>
            <address>
              <addrLine>28, rue du Docteur Roux 75724 Paris cedex 15</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-300027" type="direct"/>
            <relation name="U786" active="#struct-303623" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1330" status="OLD">
          <idno type="IdRef">134442571</idno>
          <idno type="ISNI">0000 0004 0638 7509</idno>
          <idno type="RNSR">199812904P</idno>
          <idno type="ROR">https://ror.org/02g6y2720</idno>
          <orgName>Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576</orgName>
          <orgName type="acronym">UGSF</orgName>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>Université de Lille Sciences et technologies - UMR8576 CNRS Cité Scientifique - Bâtiment C9 F-59655 Villeneuve d’Ascq Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://ugsf-umr-glycobiologie.univ-lille1.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-374570" type="direct"/>
            <relation name="UMR8576" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-18375" status="OLD">
          <orgName>Imagerie Dynamique (Plate-Forme)</orgName>
          <orgName type="acronym">PFID</orgName>
          <desc>
            <address>
              <addrLine>25-28 rue du Docteur Roux, F-75724 Paris Cedex 15</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-300027" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-229965" status="OLD">
          <orgName>Collège de France - Chaire Microbiologie et Maladies infectieuses</orgName>
          <date type="start">2008-01-01</date>
          <date type="end">2020-12-31</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-300026" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300027" status="VALID">
          <idno type="IdRef">027936643</idno>
          <idno type="ISNI">0000 0001 2353 6535</idno>
          <idno type="ROR">https://ror.org/0495fxg12</idno>
          <orgName>Institut Pasteur [Paris]</orgName>
          <orgName type="acronym">IP</orgName>
          <date type="start">1887-06-04</date>
          <desc>
            <address>
              <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.pasteur.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-374570" status="VALID">
          <idno type="IdRef">223446556</idno>
          <idno type="ISNI">0000 0001 2242 6780</idno>
          <idno type="ROR">https://ror.org/02kzqn938</idno>
          <idno type="Wikidata">Q3551621</idno>
          <orgName>Université de Lille</orgName>
          <desc>
            <address>
              <addrLine>EPE Université de Lille. -- 42 rue Paul Duez, 59000 Lille</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.univ-lille.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300026" status="VALID">
          <orgName>Collège de France</orgName>
          <orgName type="acronym">CdF (institution)</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="europeanProject" xml:id="projeurop-91946" status="VALID">
          <idno type="number">264388</idno>
          <idno type="program">FP7-PEOPLE-2010-ITN</idno>
          <idno type="call">FP7-PEOPLE-2010-ITN</idno>
          <orgName>EIMID ITN</orgName>
          <desc>European Initiative for basic research in Microbiology and Infectious Diseases</desc>
          <date type="start">2010-01-10</date>
          <date type="end">2014-09-30</date>
        </org>
        <org type="europeanProject" xml:id="projeurop-13551" status="VALID">
          <idno type="number">232798</idno>
          <idno type="program">ERC-2008-AdG</idno>
          <idno type="call">ERC-2008-AdG</idno>
          <orgName>HOMEOEPITH</orgName>
          <desc>Homeostasis and rupture of the gut epithelium in the presence of commensals and pathogens</desc>
          <date type="start">2009-01-02</date>
          <date type="end">2014-03-31</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>